bs-8687R-BF350 [Conjugated Primary Antibody]
p53 (FL-393) Polyclonal Antibody, AbBy Fluor® 350 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: p53 FL-393

Immunogen Range: 1-393/393


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7157

Swiss Prot: P04637

Source: Full length human p53 protein

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Conjugation: AbBy Fluor® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 53


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Qin, Qin, et al. "Effects of irreversible electroporation on cervical cancer cell lines in vitro." Molecular Medicine Reports 14.3 (2016): 2187-2193.Read more>>
  • Zeng, Kun, et al. "Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice." Bioscience Reports (2017): BSR20170695.Read more>>
  • Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
  • Zhan C et al. Rotenone and 3-bromopyruvate toxicity impacts electrical and structural cardiac remodeling in rats. Toxicol Lett. 2019 Oct 1. pii: S0378-4274(19)30295-4. Read more>>
  • Sola M et al. Tau affects P53 function and cell fate during the DNA damage response. Commun Biol. 2020 May 19;3(1):245.Read more>>
  • Jacquelyn Powers. et al. A Rare TP53 Mutation Predominant in Ashkenazi Jews Confers Risk of Multiple Cancers. Cancer Res. 2020 Sep;80(17):3732-3744Read more>>
  • McCann, Jennifer J.. et al. Mutant p53 elicits context-dependent pro-tumorigenic phenotypes. Oncogene. 2021 Nov;:1-15Read more>>
  • Hao, Yanan. et al. Gut microbiota-testis axis: FMT improves systemic and testicular micro-environment to increase semen quality in type 1 diabetes. MOL MED. 2022 Dec;28(1):1-17Read more>>
  • Merle Nastasja. et al. Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase. MOL CANCER. 2022 Dec;21(1):1-22Read more>>
  • Shakur Mohibi. et al. ZFP14 Regulates Cancer Cell Growth and Migration by Modulating p53 Protein Stability as Part of the MDM2 E3 Ubiquitin Ligase Complex. CANCERS. 2022 Jan;14(21):5226Read more>>
  • Hanhan Fang. et al. Gut-Spleen Axis: Microbiota via Vascular and Immune Pathways Improve Busulfan-Induced Spleen Disruption | mSphere. MSPHERE. 2022 DecRead more>>
  • Liu, Zhenni. et al. CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence. INT J BIOL SCI. 2023 Jan;19(3):950-966Read more>>
  • Yanxia Wu. et al. SCARNA10 regulates p53 acetylation-dependent transcriptional activity. BIOCHEM BIOPH RES CO. 2023 Aug;669:38Read more>>
  • Yu-Sheng Shi. et al. Melatonin Mitigates Atrazine-Induced Renal Tubular Epithelial Cell Senescence by Promoting Parkin-Mediated Mitophagy. Research. 2024 May;7Read more>>
  • Jianfang Wang. et al. Knockdown of NFIC Promotes Bovine Myoblast Proliferation through the CENPF/CDK1 Axis. J AGR FOOD CHEM. 2024;72(22):1264112654Read more>>
VALIDATION IMAGES