bs-8585R
[Primary Antibody]
Arginase 1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Arginase 1
Immunogen Range: 151-220/322
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 383
Swiss Prot: P05089
Source: KLH conjugated synthetic peptide derived from human Arginase 1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Arginase I which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea . The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster. Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location. Specifically, arginase II localizes to the mitochondria. Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver. The human arginase II gene, which maps to chromosome 14q24.1-q24.3, encodes a 354 amino acid protein. In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence.
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 35
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Sheep
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Ma, Juan, et al. "A Crucial Role of Lateral Size for Graphene Oxide in Activating Macrophages and Stimulating Pro-inflammatory Responses in Cells and Animals." ACS nano (2015).Read more>>
- Huang, Ling, et al. "Enhancement of Local Bone Remodeling in Osteoporotic Rabbits by Biomimic Multilayered Structures on Ti6Al4V Implants." Journal of Biomedical Materials Research Part A (2016).Read more>>
- EMŐKE HORVÁTH et al. FISH-OIL PRECONDITIONING UP-REGULATES EXPRESSION OF SPLENIC ARG1 POSITIVE M2 TYPE MACROPHAGES AND THE ARG1/INOS2 RATIO AFTER EXPERIMENTAL INDUCED TRANSIENT CEREBRAL ISCHEMIA. FARMACIA, 2019, Vol. 67, 5.Read more>>
- Sun Y et al. Apple polysaccharide prevents from colitis-associated carcinogenesis through regulating macrophage polarization. Int J Biol Macromol. 2020 Oct 15;161:704-711.Read more>>
- Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240.Read more>>
- Peng Yuan. et al. The nanomaterial-induced bystander effects reprogrammed macrophage immune function and metabolic profile. Nanotoxicology. 2020;14(8):1137-1155Read more>>
- Lushuang Xie. et al. Inflammatory factors and amyloid -induced microglial polarization promote inflammatory crosstalk with astrocytes.. Aging-Us. 2020 Nov 30; 12(22): 22538C22549Read more>>
- Gaoyang Guo. et al. A method for simultaneously crosslinking and functionalizing extracellular matrix-based biomaterials as bioprosthetic heart valves with enhanced endothelialization and reduced inflammation. Acta Biomater. 2021 Jan;119:89Read more>>
- Guanglin Zhang. et al. Soft apoptotic-cell-inspired nanoparticles persistently bind to macrophage membranes and promote anti-inflammatory and pro-healing effects. Acta Biomater. 2021 JulRead more>>
- Xueyang Zhao. et al. Improved tribological properties, cyto-biocompatibility and anti-inflammatory ability of additive manufactured Ti-6Al-4V alloy through surface texturing and nitriding. Surf Coat Tech. 2021 Nov;425:127686Read more>>
- Fei Chen. et al. Helminth resistance is mediated by differential activation of recruited monocyte-derived alveolar macrophages and arginine depletion. Cell Rep. 2022 Jan;38:110215Read more>>
- Juin-Hong Cherng. et al. Hemostasis and Anti-Inflammatory Abilities of AuNPs-Coated Chitosan Dressing for Burn Wounds. J PERS MED. 2022 Jul;12(7):1089Read more>>
- Xiao-Dan Li. et al. Fabricating biodegradable calcium phosphate/calcium sulfate cement reinforced with cellulose: in vitro and in vivo studies. J MATER CHEM B. 2022 NovRead more>>
- Qianhua Feng. et al. Nanoparticle cluster depolymerizes and removes amyloid fibrils for Alzheimers disease treatment. NANO TODAY. 2023 Feb;48:101756Read more>>
- Dan-dan Wu. et al. STING mediates SU5416/hypoxia-induced pulmonary arterial hypertension in rats by regulating macrophage NLRP3 inflammasome activation. IMMUNOBIOLOGY. 2023 Mar;228:152345Read more>>
- Longcong Dong. et al. Shared Genes of PPARG and NOS2 in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation. INT J MOL SCI. 2023 Jan;24(6):5651Read more>>
- Changjun Chen. et al. Engineered Exosome-Functionalized Extracellular Matrix-Mimicking Hydrogel for Promoting Bone Repair in Glucocorticoid-Induced Osteonecrosis of the Femoral Head. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
- Zhuang Cuicui. et al. Lycopene promoted M2 macrophage polarization via inhibition of NOTCH1-PI3K-mTOR-NF-B-JMJD3-IRF4 pathway in response to Escherichia coli infection in J744A.1 cells. ARCH MICROBIOL. 2024 Jun;206(6):1-11Read more>>
- Ao Wang. et al. Inhibition of NLRP3 inflammasome ameliorates LPS-induced neuroinflammatory injury in mice via PINK1/Parkin pathway. NEUROPHARMACOLOGY. 2024 Oct;257:110063Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human liver carcinoma labeled with Anti-Arginase 1 Polyclonal Antibody, Unconjugated (bs-8585R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Arginase 1 Polyclonal Antibody(bs-8585R-AF488)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).