bs-8571R [Primary Antibody]
Histone H2A.X (Ubiquityl Lys119) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Histone H2A.X Ubiquityl Lys119

Specificity: This modification site is homologous to that of Lys119 in Rat.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3014

Swiss Prot: P16104

Source: KLH conjugated synthetic peptide derived from human Ubiquityl Histone H2A.X (Lys119)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 16


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Renchao Dong. et al. Yangonin inhibits ethanol-induced hepatocyte senescence via miR-194/FXR axis. Eur J Pharmacol. 2021 Jan;890:173653Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Ubiquityl Histone H2A.X (Lys119) Polyclonal Antibody, Unconjugated (bs-8571R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: mouse brain lysates Lane 2: mouse heart lysates probed with Anti Ubiquityl Histone H2A.X (Lys119) Polyclonal Antibody, Unconjugated (bs-8571R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 16kD. Observed band size: 16kD.


Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H2A.X (Ubiquityl Lys119) Polyclonal Antibody, Unconjugated (bs-8571R) at 1:200 overnight at 4°C, DAB staining.