bs-8561R [Primary Antibody]
Laminin 2 alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Laminin 2 alpha

Immunogen Range: 2051-2200/3122


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3908

Swiss Prot: P24043

Source: KLH conjugated synthetic peptide derived from human Laminin 2 alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 341


Cross Reactive Species: Human
Mouse
Rat
Bovine
Horse

Predicted Cross Reactive Species: Dog
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Silva Nunes Barreto, Rodrigo, et al. "Decellularized bovine cotyledons may serve as biological scaffolds with preserved vascular arrangement." Journal of Tissue Engineering and Regenerative Medicine (2017).Read more>>
  • Palma et al. Equine lung decellularization: a potential approach for in vitro modeling the role of the extracellular matrix in asthma. (2018) J.Tissue.Eng. 9:2041731418810164Read more>>
  • Borghesi J et al. Characterization of the extracellular matrix in the chorioallantoic membrane of water buffalo (Bubalus bubalis) in early gestation. Reprod Domest Anim. 2019 Jul 6. Read more>>
  • Zhang, Zhao. et al. Development of a CAFs-related gene signature to predict survival and drug response in bladder cancer. Hum Cell. 2022 Jan;:1-16Read more>>
  • R S N Barreto. et al. THE EXTRACELLULAR MATRIX PROTEIN PATTERN IN THE CANINE NEOPLASTIC MAMMARY GLAND. TISSUE CELL. 2023 Feb;:10205Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Rabbit Anti LAMA2 Polyclonal Antibody, Unconjugated (bs-8561R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Laminin 2 alpha) Polyclonal Antibody, Unconjugated (bs-8561R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Laminin 2 alpha) Polyclonal Antibody, Unconjugated (bs-8561R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.