bs-8554R [Primary Antibody]
Hemoglobin Beta/Delta Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HBB + HBD

Specificity: Antibody has equal affinity for Human Hemoglobin Beta and Delta

Immunogen Range: 51-147/147


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3043; 3045

Swiss Prot: P68871; P02042

Source: KLH conjugated synthetic peptide derived from human Hemoglobin beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The delta (HBD) and beta (HBB) genes are normally expressed in the adult: two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin. Two alpha chains plus two delta chains constitute HbA-2, which with HbF comprises the remaining 3% of adult hemoglobin. Five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon--Ggamma--Agamma--delta--beta-3'. Mutations in the delta-globin gene are associated with beta-thalassemia.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 15.5


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: K562 Cells; Probed with Hemoglobin Beta Polyclonal Antibody (bs-8554R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.