bs-8551R-Cy5 [Conjugated Primary Antibody]
Nitrotyrosine Polyclonal Antibody, Cy5 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Nitrotyrosine

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated to Nitrotyrosine

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure.

Conjugation: Cy5

Excitation/ Emission: 625,650nm/670nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Others
(Nitrotyrosine)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Vendrov, Aleksandr, et al. "NOX4 NADPH Oxidase-dependent Mitochondrial Oxidative Stress in Aging-associated Cardiovascular Disease." Antioxidants and Redox Signaling ja (2015).Read more>>
  • Chen, Yu-Lin, and Wai-Ming Kan. "Down-regulation of superoxide dismutase 1 by PMA is involved in cell fate determination and mediated via protein kinase D2 in myeloid leukemia cells." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research (2015).Read more>>
  • Wang, Lin-Feng, et al. "Tert-butylhydroquinone ameliorates doxorubicin-induced cardiotoxicity by activating Nrf2 and inducing the expression of its target genes." American Journal of Translational Research 7.10 (2015): 1724-1735.Read more>>
  • Li, Dawei, et al. "Hepatic Hypoxia-Inducible Factors Inhibit PPARα Expression To Exacerbate Acetaminophen induced Oxidative Stress And Hepatotoxicity." Free Radical Biology and Medicine (2017).Read more>>
  • Li et al. Targeting intestinal epithelial cell-programmed necrosis alleviates tissue injury after intestinal ischemia/reperfusion in rats. (2018) J.Surg.Res. 225:108-117Read more>>
  • Canonica J et al. Effect of acute and chronic aldosterone exposure on the retinal pigment epithelium-choroid complex in rodents. Exp Eye Res. 2019 Aug 5;187:107747.Read more>>
  • Feng Y et al. Methane Alleviates Acetaminophen-Induced Liver Injury by Inhibiting Inflammation, Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis through the Nrf2/HO-1/NQO1 Signaling Pathway. Oxid Med Cell Longev. 2019 Nov 6;2019:7067619.Read more>>
  • Ahmed S et al. Cocoa Flavonoids Reduce Inflammation and Oxidative Stress in a Myocardial Ischemia-Reperfusion Experimental Model. Antioxidants (Basel). 2020 Feb 18;9(2). pii: E167.Read more>>
  • Yan Xuet al. Blockade of Platelets Using Tumor-Specific NO-Releasing Nanoparticles Prevents Tumor Metastasis and Reverses Tumor Immunosuppression. ACS Nano. 2020 Aug 25;14(8):9780-9795.Read more>>
  • Chuang Yang. et al. PPAR down-regulation is involved in high glucose-induced endothelial injury via acceleration of nitrative stress. Microvasc Res. 2022 Jan;139:104272Read more>>
  • Yuan Chen. et al. Intrinsic Radical Species Scavenging Activities of Tea Polyphenols Nanoparticles Block Pyroptosis in Endotoxin-Induced Sepsis. Acs Nano. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Shikai Liu. et al. On-Demand Generation of Peroxynitrite from an Integrated Two-Dimensional System for Enhanced Tumor Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Chuang Yang. et al. Gastrodin protects endothelial cells against high glucose-induced injury through up-regulation of PPAR and alleviation of nitrative stress. MICROVASC RES. 2023 Jul;148:104531Read more>>
  • Aleyna ?ztzn. et al. Inflammatory signal transduction pathways induced by prilocaine toxicity in cultured ARPE-19 cells. J BIOCHEM MOL TOXIC. 2023 Aug;:e23491Read more>>
  • Siyuan Wang. et al. Inhibition of cisplatin-induced Acsl4-mediated ferroptosis alleviated ovarian injury. CHEM-BIOL INTERACT. 2024 Jan;387:110825Read more>>
  • Xu-Rui Gu. et al. A renal-targeted gene delivery system derived from spermidine for arginase-2 silencing and synergistic attenuation of drug-induced acute kidney injury. CHEM ENG J. 2024 Apr;486:150125Read more>>
  • Zhou Yi. et al. The value of low-intensity pulsed ultrasound in reducing ovarian injury caused by chemotherapy in mice. REPROD BIOL ENDOCRIN. 2024 Dec;22(1):1-15Read more>>
  • Zhang Fangfang. et al. GFRA4 improves the neurogenic potential of enteric neural crest stem cells via hedgehog pathway. PEDIATR RES. 2024 Apr;:1-9Read more>>
  • Li Fangfang. et al. Icariin alleviates cisplatin-induced premature ovarian failure by inhibiting ferroptosis through activation of the Nrf2/ARE pathway. SCI REP-UK. 2024 Jul;14(1):1-17Read more>>
  • Zhenyu Feng. et al. Cardamonin intervenes in myocardial hypertrophy progression by regulating Usp18. PHYTOMEDICINE. 2024 Nov;134:15597Read more>>
VALIDATION IMAGES