bs-8544R [Primary Antibody]
GABR B3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GABR B3

Specificity: Possible cross-reactivity with GABRB1 and GABRB2

Immunogen Range: 31-130/473


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2562

Swiss Prot: P28472

Source: KLH conjugated synthetic peptide derived from human GABR B3/GABA A Receptor beta 3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 52


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse Cerebrum lysates probed with GABRB3 Polyclonal Antibody, unconjugated (bs-8544R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Lane 1: Mouse Cerebrum, Lane 2: Mouse Hippocampus; Probed with GABRB3 Polyclonal Antibody, unconjugated (bs-8544R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:20000 for 90 minutes at 37°C.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with GABR B3 Polyclonal Antibody, Unconjugated (bs-8544R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.