bs-8538R [Primary Antibody]
Prostaglandin E Receptor EP4 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Prostaglandin E Receptor EP4

Immunogen Range: 151-250/488


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5734

Swiss Prot: P35408

Source: KLH conjugated synthetic peptide derived from human EP4R/Prostaglandin E Receptor EP4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 53


Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hu, Ruiqi, et al. "The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii)." Prostaglandins & Other Lipid Mediators (2016).Read more>>
  • Takemiya et al. Microsomal Prostaglandin E Synthase-1 Facilitates an Intercellular Interaction between CD4⁺ T Cells through IL-1β Autocrine Function in Experimental Autoimmune Encephalomyelitis. (2017) Int.J.Mol.Sci. 18 Read more>>
  • Wang Y et al. Seasonal expressions of COX-1, COX-2 and EP4 in the uteri of the wild Daurian ground squirrels (Spermophilus dauricus). Prostaglandins Other Lipid Mediat. 2019 Aug;143:106343.Read more>>
  • Xi He. et al. Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche. Cell Rep. 2021 Sep;36:109674Read more>>
  • Seung-Yub Song. et al. Purification of phenoloxidase from Haliotis discus hannai and its anti-inflammatory activity in vitro. FISH SHELLFISH IMMUN. 2023 Jun;137:108741Read more>>
  • Xin Wang. et al. Irreversible Electroporation Improves Tendon Healing in a Rat Model of Collagenase-Induced Achilles Tendinopathy. AM J SPORT MED. ;()Read more>>
  • Haojie Chen. et al. Association Between Vertebral Endplate Defects and Patient-reported Symptoms: An Immunohistochemical Study Investigating the COX-2/PGE-2/EP-4 Axis. SPINE J. 2024 ApRead more>>
  • Yi Yunhua. et al. Progressively Diminished Prostaglandin E2 Signaling in Concordance with Increasing Fibrosis in Ectopic Endometrium. REPROD SCI. 2024 Aug;:1-16Read more>>
VALIDATION IMAGES

Raji cell lysates probed with EP4 Polyclonal Antibody, Unconjugated (bs-8538R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Uterus tissue lysates; Lane 2: Mouse Small intestine tissue lysates; Lane 3: Mouse Kidney tissue lysates ; Lane 4: Mouse Prostate tissue lysates probed with EP4 Polyclonal Antibody, Unconjugated (bs-8538R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.


Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Rat Esophagus tissue lysates; Lane 4: Rat Liver tissue lysates probed with EP4 Polyclonal Antibody, Unconjugated (bs-8538R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.