bs-8533R [Primary Antibody]
Vimentin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Vimentin

Specificity: KO-Validated

Immunogen Range: 371-466/466


Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human Vimentin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 51


Cross Reactive Species: Human
Mouse
Rat
Rabbit

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang, Ning, et al. "Overexpression of SOX2 promotes migration, invasion, and epithelial-mesenchymal transition through the Wnt/β-catenin pathway in laryngeal cancer Hep-2 cells." Tumor Biology (2014): 1-9.Read more>>
  • He, D., et al. "Enhanced M1/M2 Macrophage Ratio Promotes Orthodontic Root Resorption." Journal of Dental Research (2014): 0022034514553817.Read more>>
  • Liu et al. MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line. (2017) Oncol.Let. 14:1780-1786Read more>>
  • Pan et al. MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (2017) Exp.Ther.Med. 14:4663-4670Read more>>
  • Zhou et al. Abnormal expression of fibrosis markers, estrogen receptor α and stromal derived factor‑1/chemokine (C‑X‑C motif) receptor‑4 axis in intrauterine adhesions. (2018) Int.J.Mol.Med. 42:81-90Read more>>
  • Han LL et al. miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2. (2018) Int J Oncol. Dec; 53(6): 2433–2444.Read more>>
  • Guo F et al. miR-375-3p/YWHAZ/β-catenin axis regulates migration, invasion, EMT in gastric cancer cells.(2018) Clin Exp Pharmacol Physiol. Oct 24.Read more>>
  • Liu SQ et al. Sphingosine kinase 1 promotes the metastasis of colorectal cancer by inducing the epithelial‑mesenchymal transition mediated by the FAK/AKT/MMPs axis.Int. J. Oncol. 2018 Oct 25Read more>>
  • Zhou Q et al. Abnormal expression of fibrosis markers, estrogen receptor α and stromal derived factor-1/chemokine (CXC motif) receptor-4 axis in intrauterine adhesions. Int. J. Mol. Med. 2018 Jul;42(1)：81-90.Read more>>
  • Wu Y et al. MFAP5 promotes basal-like breast cancer progression by activating the EMT program.Cell Biosci. 2019 Mar 7;9:24. Read more>>
  • Liu YR et al. Long noncoding RNA MEG3 regulates rheumatoid arthritis by targeting NLRC5. J Cell Physiol. 2019 Aug;234(8):14270-14284.Read more>>
  • Li D et al. Oxygenated Polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitate lung cancer metastasis by epigenetically regulating the epithelial-to-mesenchymal transition (EMT). Environ Pollut. 2019 Sep 17;255(Pt 2):113261. Read more>>
  • Le G et al. Ochratoxin A induces glomerular injury through activating the ERK/NF-B signaling pathway. Food Chem Toxicol. 2020 Sep;143:111516.Read more>>
  • Liu H et al. Anti-tubulin agent vinorelbine inhibits metastasis of cancer cells by regulating epithelial-mesenchymal transition. Eur J Med Chem. 2020 Aug 15;200:112332.Read more>>
  • Yun Liu. et al. Everolimus reduces postoperative arthrofibrosis in rabbits by inducing autophagy-mediated fibroblast apoptosis by PI3K/Akt/mTOR signaling pathway. Biochem Bioph Res Co. 2020 Nov;533:1Read more>>
  • Jing Guo. et al. The association between RGS4 and choline in cardiac fibrosis. Cell Commun Signal. 2021 Dec;19(1):1-13Read more>>
  • Jinzhu Han. et al. Circ_0027599 elevates RUNX1 expression via sponging miR-21-5p on gastric cancer progression. 2021 May 25Read more>>
  • Mei Tao. et al. Tripterygium glycoside suppresses epithelial?to?mesenchymal transition of diabetic kidney disease podocytes by targeting autophagy through the mTOR/Twist1 pathway. Mol Med Rep. 2021 Aug;24(2):1-11Read more>>
  • oyu Chen. et al. Study of the effects of rabbit scleral fibroblasts on cellular biomechanical properties and MMP-2 expression using two modes of riboflavin/ultraviolet A wave collagen cross-linking. Exp Eye Res. 2021 Jul;:108695Read more>>
  • Liu Yujuan. et al. Cysteine-Rich Intestinal Protein 1 Served as an Epithelial Ovarian Cancer Marker via Promoting Wnt/-Catenin-Mediated EMT and Tumour Metastasis. Dis Markers. 2021;2021:3566749Read more>>
  • Guannan Le. et al. Combination of zinc and selenium alleviates ochratoxin A-induced fibrosis via blocking ROS-dependent autophagy in HK-2 cells. J Trace Elem Med Bio. 2022 Jan;69:126881Read more>>
  • Longfei Xiao. et al. Dihydrotestosterone through blockade of TGF-/Smad signaling mediates the anti-fibrosis effect under hypoxia in canine Sertoli cells. J Steroid Biochem. 2022 Feb;216:106041Read more>>
  • Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11Read more>>
  • Tian, Mengyue. et al. The protective effect of licochalcone A against inflammation injury of primary dairy cow claw dermal cells induced by lipopolysaccharide. Sci Rep-Uk. 2022 Jan;12(1):1-9Read more>>
  • Yanqiu Sun. et al. Overexpression of long noncoding RNA LINC00638 inhibits inflammation and oxidative stress in rheumatoid arthritis fibroblast-like synoviocytes by regulating the Nrf2/HO-1 pathway. IMMUN INFLAMM DIS. 2022 Jul;10(7):e663Read more>>
  • Xiaohong Yu. et al. Effects of Yunnan Baiyao on the Differentiation of HPDLFs on the Bio-Oss Collagen Scaffold in vivo. INT J GEN MED. 2022; 15: 53955405Read more>>
  • Zihui Wang. et al. Heat stress and hypoxia inhibit the secretion of androgens and induce epithelial-to-mesenchymal transition associated with activated TGF-_/Smad signaling in canine cryptorchidism. REPROD DOMEST ANIM. 2022 Jun 09Read more>>
  • Shan Huang. et al. Effects of Ocular Hypertension on Cytoskeleton and Stiffness of Trabecular Meshwork Cells in Rats. APPL SCI-BASEL. 2022 Jan;12(15):7862Read more>>
  • Guannan Le. et al. Ochratoxin A induced differentiation nephrotoxicity in renal tubule and glomeruli via autophagy differential regulation. ENVIRON TOXICOL PHAR. 2022 Oct;95:103973Read more>>
  • Cheng He. et al. Crosstalk of renal cell carcinoma cells and tumor-associated macrophages aggravates tumor progression by modulating muscleblind-like protein 2/B-cell lymphoma 2/beclin 1-mediated autophagy. CYTOTHERAPY. 2022 OcRead more>>
  • Shu Qing Liu. et al. Depletion of Tgfbr2 in hepatocytes alleviates liver fibrosis and restores hepatic function in fibrotic mice. J DIGEST DIS. 2023 FebRead more>>
  • Yang Dapeng. et al. Morphological and histochemical identification of telocytes in adult yak epididymis. SCI REP-UK. 2023 Mar;13(1):1-14Read more>>
  • aosheng Zhou. et al. ADAMTS8 inhibits glioma development in vitro and in vivo. FOLIA NEUROPATHOL. 2023 Jul;61(2):144-152Read more>>
  • Nozima Aripova. et al. Citrullinated and malondialdehyde-acetaldehyde modified fibrinogen activates macrophages and promotes an aggressive synovial fibroblast phenotype in patients with rheumatoid arthritis. FRONT IMMUNOL. 2023; 14: 1203548Read more>>
  • Jingxin Li. et al. A Mammalian Conserved Circular RNA CircLARP1B Regulates Hepatocellular Carcinoma Metastasis and Lipid Metabolism. Advanced Science. 2023 Nov;:2305902Read more>>
  • Guan Wang. et al. LncRNA HOTTIP regulates TLR4 promoter methylation by recruiting H3K4 methyltransferase MLL1 to affect apoptosis and inflammatory response of fibroblast-like synoviocyte in rheumatoid arthritis. KAOHSIUNG J MED SCI. 2024 FebRead more>>
  • Wang Bin. et al. Enhancing endometrial receptivity: the roles of human chorionic gonadotropin in autophagy and apoptosis regulation in endometrial stromal cells. REPROD BIOL ENDOCRIN. 2024 Dec;22(1):1-18Read more>>
  • Fu Rongrong. et al. SOAT1 regulates cholesterol metabolism to induce EMT in hepatocellular carcinoma. CELL DEATH DIS. 2024 May;15(5):1-14Read more>>
  • Wang Jianhang. et al. Genistein promotes cartilage repair and inhibits synovial inflammatory response after anterior cruciate ligament transection in rats by regulating the Wnt/-catenin axis. N-S ARCH PHARMACOL. 2024 May;:1-16Read more>>
  • Yuan Zhao. et al. Dextran sulfate inhibits proliferation and metastasis of human gastric cancer cells via miR-34c-5p. HELIYON. 2024 Aug;1Read more>>
  • Ting Chen. et al. Investigation roles of Adamts1 and Adamts5 in scleral fibroblasts under hypoxia and mice with form-deprived myopia. EXP EYE RES. 2024 Oct;247:110026Read more>>
VALIDATION IMAGES

4% Paraformaldehyde-fixed Human Umbilical Artery Endothelial Cells(HUAEC)probed with Anti-Vimentin Polyclonal Antibody, Alexa Fluor 488 conjugated (bs-8533R-A488) 1:100, 60 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei


Lymph node lysates probed with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Hela KO Vimentin cell lysates; Lane 2: Hela cell lysates probed with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: U-87MG cell lysates; Lane 2: Mouse NIH/3T3 cell lysates probed with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Tissue/cell: U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-8533R)antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Vimentin Polyclonal Antibody(bs-8533R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Lane 1: Human A549 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human U251 cell lysates probed with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-8533R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Vimentin polyclonal antibody, unconjugated (bs-8533R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.