bs-8510R [Primary Antibody]
XRCC4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: XRCC4

Immunogen Range: 151-260/336


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7518

Swiss Prot: Q13426

Source: KLH conjugated synthetic peptide derived from human XRCC4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 38/60


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Talibova, Gunel. et al. Increased double-strand breaks in aged mouse male germ cells may result from changed expression of the genes essential for homologous recombination or nonhomologous end?joining repair. HISTOCHEM CELL BIOL. 2022 Oct;:1-21Read more>>
  • Talibova Gunel. et al. The DNA double-strand break repair proteins H2AX, RAD51, BRCA1, RPA70, KU80, and XRCC4 exhibit follicle-specific expression differences in the postnatal mouse ovaries from early to older ages. J ASSIST REPROD GEN. 2024 Jul;:1-21Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat colitis labeled with Anti XRCC4 Polyclonal Antibody, Unconjugated (bs-8510R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Molt-4 cell lysates probed with XRCC4 Polyclonal Antibody, Unconjugated (bs-8510R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.