DATASHEET
Host:
Rabbit
Target Protein:
Tau protein Thr181
Modification Site:
Thr181
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
4137
Source:
KLH conjugated synthetic phosphopeptide derived from human Tau protein around the phosphorylation site of Thr181
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Tau proteins are important Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Tau proteins subcellular located in the axons of neurons, in the cytoso l and in association with plasma membrane components. It expressed in neurons. PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
VALIDATION IMAGES
Lane 1: Mouse Cerebrum cell lysates; Lane 2: Rat Cerebrum cell lysates; Lane 3: Mouse Cerebellum cell lysates; Lane 4: Rat Cerebellum cell lysates probed with Tau protein(Thr181) Polyclonal Antibody, Unconjugated (bs-8453R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tau protein(Thr181) Polyclonal Antibody, Unconjugated (bs-5421R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tau protein(Thr181) Polyclonal Antibody, Unconjugated (bs-5421R) at 1:200 overnight at 4°C, DAB staining.