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Host: Rabbit
Target Protein: Properdin
Immunogen Range: 401-469/469
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5199
Swiss Prot: P27918
Source: KLH conjugated synthetic peptide derived from human Properdin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 48
Predicted Cross Reactive Species:
Human
Mouse
Rat
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Nashine et al. Differential Expression of Complement Markers in Normal and AMD Transmitochondrial Cybrids. (2016) PLoS.On. 11:e0159828Read more>>
- Chen C et al. Alternative complement pathway is activated in the brains of scrapie-infected rodents. Med Microbiol Immunol. 2019 Nov 12.Read more>>
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Properdin Polyclonal Antibody, Unconjugated (bs-8306R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Properdin Polyclonal Antibody, Unconjugated (bs-8306R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Properdin Polyclonal Antibody, Unconjugated (bs-8306R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Properdin Polyclonal Antibody, Unconjugated (bs-8306R) at 1:100 overnight at 4°C, followed by secondary and DAB staining.