Host: Rabbit
Target Protein: Alpha casein
Immunogen Range: 31-130/185
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1446
Source: KLH conjugated synthetic peptide derived from human Alpha casein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 28
Cross Reactive Species:
Human
Mouse
Bovine
For research use only. Not intended for diagnostic or therapeutic use.
- Chu, Meiqiang, et al. "MicroRNA-126 participates in lipid metabolism in mammary epithelial cells." Molecular and Cellular Endocrinology (2017).Read more>>
- Qi H et al. Methionine Promotes Milk Protein and Fat Synthesis and Cell Proliferation via the SNAT2-PI3K Signaling Pathway in Bovine Mammary Epithelial Cells.(2018) J Agric Food Chem.66(42):11027-11033.Read more>>
- Huo N et al. PURB is a positive regulator of amino acid‐induced milk synthesis in bovine mammary epithelial cells.(2018) J Cell Physiol. Oct 26. Read more>>
- Yu M et al. Taurine promotes milk synthesis via the GPR87-PI3K-SETD1A signaling in BMECs. J Agric Food Chem. 2019 Feb 20;67(7):1927-1936. Read more>>
- Mengmeng Yu. et al. Daidzein promotes milk synthesis and proliferation of mammary epithelial cells via the estrogen receptor -dependent NFB1 activation. 2020 May 13Read more>>
Human breast milk Lysate Lane 1: 2 uL; Lane 2: 6 uL; Lane 3: 8uL probed with Alpha casein Polyclonal Antibody, Unconjugated (bs-8245R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Milk lysates probed with Alpha casein Polyclonal Antibody, Unconjugated (bs-8245R) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.