Host: Rabbit
Target Protein: SIPA1L2
Immunogen Range: 251-350/1722
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 57568
Swiss Prot: A0A6Q8PH54
Source: KLH conjugated synthetic peptide derived from human SIPA1L2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-SIPA1L2 Polyclonal Antibody, Unconjugated (bs-7927R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.