bs-7867R [Primary Antibody]
Iroquois homeobox protein 3 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Iroquois homeobox protein 3

Immunogen Range: 401-501/501


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 79191

Swiss Prot: P78415

Source: KLH conjugated synthetic peptide derived from human Iroquois homeobox protein 3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 52


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: mouse brain lysates Lane 2: mouse heart lysates probed with Anti Iroquois homeobox protein 3 Polyclonal Antibody, Unconjugated (bs-7867R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 52-55kD. Observed band size: 54kD.


Mouse heart lysates probed with Iroquois homeobox protein 3 Polyclonal Antibody, Unconjugated (bs-7867R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.