bs-7750R [Primary Antibody]
MKLP2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MKLP2

Immunogen Range: 353-450/890


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10112

Swiss Prot: O95235

Source: KLH conjugated synthetic peptide derived from human KIF20A/MKLP2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 100


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jie Jiang. et al. Identification of Hub Genes Associated With Melanoma Development by Comprehensive Bioinformatics Analysis. Front Oncol. 2021; 11: 62143Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti KIF20A/MKLP2 Polyclonal Antibody, Unconjugated (bs-7750R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti KIF20A/MKLP2 Polyclonal Antibody, Unconjugated (bs-7750R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Antigen: bs-7750P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450