bs-7723R [Primary Antibody]
REC8 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: REC8

Immunogen Range: 22-120/547


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9985

Swiss Prot: O95072

Source: KLH conjugated synthetic peptide derived from human REC8

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 60


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shimoi G et al.Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes.(2018)J Reprod Dev. Read more>>
  • Shangren Wang. et al. Long noncoding RNAs regulated spermatogenesis in varicocele-induced spermatogenic dysfunction. 2022 Mar 17Read more>>
VALIDATION IMAGES

HT-29 cell lysates probed with REC8 Polyclonal Antibody, Unconjugated (bs-7723R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.