Host: Rabbit
Target Protein: DNase 2 alpha
Immunogen Range: 25-90/360
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1777
Swiss Prot: O00115
Source: KLH conjugated synthetic peptide derived from human DNase II alpha
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 38
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Takahashi et al. Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells. (2018) Nat.Commun. 9:1249Read more>>
Paraformaldehyde-fixed, paraffin embedded mouse kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-DNase II Polyclonal Antibody, Unconjugated (bs-7652R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining\n
Paraformaldehyde-fixed, paraffin embedded mouse stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-DNase II Polyclonal Antibody, Unconjugated (bs-7652R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining\n
Human colon cancer 4% Paraformaldehyde-fixed and paraffin-embedded.\nAntigen retrieval: Citrate buffer ( 0.01M, pH 6.0 ) boiling bath for 15min; Blocking buffer (normal goat serum 10%) at 37℃ for 20 min;\nIncubation: Anti-DNase II Polyclonal Antibody, Unconjugated(bs-7652R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei\n
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-DNase II Polyclonal Antibody, Unconjugated (bs-7652R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-DNase II Polyclonal Antibody, Unconjugated (bs-7652R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.