bs-75933C

DATASHEET

Formulation Powder

Storage: Stable for 2 years after receipt when stored at -20°C.

Product Information:

CAS Number: 72040-63-2

Molecular formula: C20H30N4O6S

Molecular weight: 454.54

Purity: >97% (NMR)

Appearance: White to off-white powder.

Solubility: Soluble in DMSO (20mg/ml), DMF(20mg/ml) or methanol.

InChiKey: UVGHPGOONBRLCX-NJSLBKSFSA-N

SMILES: O=C(NCCCCCC(ON1C(CCC1=O)=O)=O)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O

Description:

Biotin-X-NHS is an amino-reactive biotinylation reagent with a long spacer arm. The 6-aminocaproic acid spacer arm provides distance between the biotin and the target ligand which helps alleviate steric hindrance. Biotin-X-NHS is used to biotinylate antibodies, proteins, peptides, or amino acids by reacting with primary amines as found in the N-terminus and Lysine side chains. The Biotin-X-NHS molecule is cell membrane permeable and can be used to label intracellular proteins. Optimal reaction conditions are at a pH of 7-9. Avoid amine-containing buffers, which may compete in the acylation reaction.