DATASHEET
Formulation
Liquid
Storage:
Stable for 2 years after receipt when stored at -20°C.
Product Information:
CAS Number: 254109-22-3
Molecular formula: C25H18F2N2O7
Molecular weight: 496.42
Purity: >98% (HPLC)
Appearance: Clear colourless liquid.
Solubility: Soluble in DMSO.
InChiKey: BEVHTVRRVVEMEF-UHFFFAOYSA-N
SMILES: CNC1=C(N)C2=C(C=C1)C1(OC2=O)C2=C(OC3=C1C=C(F)C(OC(C)=O)=C3)C=C(OC(C)=O)C(F)=C2
Description:
DAF-FM diacetate is a sensitive cell-permeable fluorescent probe important reagent for quantification of low concentrations of nitric oxide in solution. More photo-stable and less pH sensitive than DAF-2.This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM diacetate permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument suitable for detecting fluorescein.