bs-75807C

DATASHEET

Formulation Solid

Storage: Stable for 2 years after receipt when stored at RT.

Product Information:

CAS Number: 53518-15-3

Molecular formula: C10H6F3NO2

Molecular weight: 229.16

Purity: >99% (GC)

Appearance: Yellow solid.

Solubility: Soluble in DMSO, DMF or methanol (all 25mg/ml). Insoluble in water.

InChiKey: JBNOVHJXQSHGRL-UHFFFAOYSA-N

SMILES: NC1=CC=C2C(OC(=O)C=C2C(F)(F)F)=C1

Description:

7-Amino-4-trifluoromethylcoumarin (AFC) is used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids and therefore the precursor for a variety of AFC enzyme substrates. It is a fluorogenic standard useful as a calibration standard for AFC-based enzyme substrates and for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin (AFC) release such as those utilizing ubiquitin-AFC. Compared to AMC enzyme substrates, fluorogenic AFC substrates generate AFC that has longer absorption and fluorescence wavelength that AMC. AFC is also suitable as laser dye. Spectral Data: lambdaex 376nm, lambdaem 482nm, Quantum yield 0.531.