bs-75479C

DATASHEET

Formulation Powder

Storage: Stable for 2 years after receipt when stored at -20°C.

Product Information:

CAS Number: 3520-43-2 and 47729-63-5

Molecular formula: C25H27Cl4IN4

Molecular weight: 652.2

Purity: >98% (NMR)

Appearance: Red powder.

Solubility: Soluble in DMSO, ethanol, methanol or acetone.

InChiKey: FYNNIUVBDKICAX-UHFFFAOYSA-M

SMILES: [I-].CCN1C(=CC=CC2=[N+](CC)C3=C(C=C(Cl)C(Cl)=C3)N2CC)N(CC)C2=C1C=C(Cl)C(Cl)=C2

Description:

The membrane-permeant dual-emission potential-sensitive JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-1 dye can be used as an indicator of mitochondrial membrane potential in a variety of cell types, including myocytes and neurons, as well as in intact tissues and isolated mitochondria. JC-1 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. After cell injury, as membrane potential decreases, JC-1 monomers are generated, resulting in a shift to green emission (529 nm). The principal advantage of JC-1 relative to other commonly employed fluorescent probes of mitochondrial membrane potential is that it allows qualitative visualization, considering the shift from orange to green fluorescence emission, and quantitative detection, considering the fluorescence intensity ratio.