bs-7537R [Primary Antibody]
SIRT4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SIRT4

Immunogen Range: 51-150/314


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23409

Swiss Prot: Q9Y6E7

Source: KLH conjugated synthetic peptide derived from human SIRT4/SIR 2 like protein 4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Acts both as NAD-dependent protein ADP-ribosyl transferase and NAD-dependent protein deacetylase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Down-regulates insulin secretion.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 32


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Cow
Sheep
Pig
Horse
Rabbit
Zebrafish
Fruit Fly

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Takumida, Masaya, Hiroshi Takumida, and Matti Anniko. "Localization of sirtuins in the mouse inner ear." Acta Oto-Laryngologica 0 (2014): 1-8.Read more>>
  • Takumida, Masaya, et al. "Localization of sirtuins (SIRT1-7) in the aged mouse inner ear." Acta oto-laryngologica (2015): 1-12.Read more>>
  • Chih-Chang Chao. et al. SRT1720 as an SIRT1 activator for alleviating paraquat-induced models of Parkinson's disease. REDOX BIOL. 2022 Dec;58:102534Read more>>
  • Ai-Wen Kang. et al. Puerarin extends the lifespan of Drosophila melanogaster by activating autophagy. FOOD FUNCT. 2023 FebRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-SIRT4 Polyclonal Antibody, Unconjugated (bs-7537R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining\n


Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-SIRT4 Polyclonal Antibody, Unconjugated (bs-7537R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining\n


Mouse cerebrum lysates probed with SIRT4 Polyclonal Antibody, unconjugated (bs-7537R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n


Mouse liver lysates probed with SIRT4 Polyclonal Antibody, unconjugated (bs-7537R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.