DATASHEET
Formulation
Solid
Storage:
Stable for 1 year after receipt when stored at -20°C.
Product Information:
CAS Number: 62354-43-2
Molecular formula: C23H38N6O5 . 2HCl
Molecular weight: 478.6 . 72.9
Purity: >98% (HPLC)
Appearance: White to off-white solid.
Solubility: Soluble in water (50mg/ml).
InChiKey: VESQMNNSPPEOSZ-ZLARAOTRSA-N
SMILES: [H]Cl.[H]Cl.O=C(N[C@@H](CCCCN)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)[C@H](CC(C)C)NC([C@@H](C(C)C)N)=O
Description:
D-Val-Leu-Lys-p-nitroanilide is a selective colorimetric substrate for plasmin used to determine the formation of plasmin from plasminogen in amidolytic activity assays and plasminogen activating assays. Plasmin is a plasma serine protease whose main role is to dissolve fibrin blood clots. After cleavage by plasmin the protease activity is quantified by the release of p-nitroaniline (pNA) from the substrate. The rate of pNA formation, e.g. the increase in absorbance per second at 405 nm, is proportional to the enzymatic activity.