Host: Rabbit
Target Protein: ATG14
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 22863
Swiss Prot: Q6ZNE5
Source: KLH conjugated synthetic peptide derived from human ATG14
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Predicted Molecular Weight: 55
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG14) Polyclonal Antibody, Unconjugated (bs-7462R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human A431 cell lysates probed with ATG14 Polyclonal Antibody, Unconjugated (bs-7462R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.