Host: Rabbit
Target Protein: CD200R2
Immunogen Range: 151-250/325
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 344807
Swiss Prot: Q6Q8B3
Source: KLH conjugated synthetic peptide derived from human CD200R2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 34
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
- Clark, David A., Jorge L. Arredondo, and Sukhbinder Dhesy-Thind. "The CD200 tolerance-signaling molecule and its receptor, CD200R1, are expressed in human placental villus trophoblast and in peri-implant decidua by 5 weeks’ gestation." Journal of Reproductive Immunology 112 (2015): 20-23.Read more>>
- Clark, David A., et al. "The Receptor for the CD200 Tolerance‐Signaling Molecule Associated with Successful Pregnancy is Expressed by Early‐Stage Breast Cancer Cells in 80% of Patients and by Term Placental Trophoblasts." American Journal of Reproductive Immunology 74.5 (2015): 387-391.Read more>>
- Clark, David A., et al. "Changes in expression of the CD200 tolerance‐signaling molecule and its receptor (CD200R) by villus trophoblasts during first trimester missed abortion and in chronic histiocytic intervillositis." American Journal of Reproductive Immunology (2017).Read more>>
U937 cell lysates probed with Rabbit Anti-CD200R2 Polyclonal Antibody, Unconjugated (bs-7351R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD200R2 Polyclonal Antibody, Unconjugated (bs-7351R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
HepG2 cells probed with Rabbit Anti-CD200R2 Polyclonal Antibody, A647 Conjugated (bs-7351R-A647) at 5ug for 30 minutes (green) compared to control cells (blue) and isotype control (orange).
HepG2 cells probed with CD200R2 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-7351R-A647) at 1:100 for 30 minutes compared to control cells (blue)and isotype control (orange).
Staining of Placental villus from 7w gestation karyotype-normal pregnancy with bs-7351R. The Positive Control used is Placental villus from 7w gestation karyotype-normal pregnancy. The Negative Control used was KLH antibody. Images were taken at 400x magnification, bars represent 60µm. Figure legend: VC: villus core, CT: cytotrophoblast, ST: syncytiotrophoblast, MB: maternal blood space bathing placental villi. Image was kindly submitted by a researcher via the Antibodies Online Independent Validation Program.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD200R2 Polyclonal Antibody, Unconjugated (bs-7351R) at 1:400 overnight at 4°C, DAB staining.