bs-70725R

DATASHEET

Host: Rabbit

Target Protein: N-WASP

Specificity: This antibody detects a 65 kDa* protein corresponding to the molecular mass of N-WASP on SDS-PAGE immunoblots of neonatal rat brain lysate. It is also detects 65 kDa* proteins in A431, human endothelial, and SKN-SH cells. It does not recognize the 63 kDa* WASP protein in Jurkat cell lysate. This N-WASP peptide sequence is 100% homologous to rat and mouse N-WASP, and has low homology to the corresponding region in the human WASP.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: O00401

Source: N-WASP synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human N-WASP.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain.