bs-70714R

DATASHEET

Host: Rabbit

Target Protein: VASP (Thr-278)

Specificity: The antibody detects a 50 kDa* band corresponding to VASP in Western blots of human A431 and HeLa cells, as well as mouse C2C12 cells that have been treated with calyculin A. This sequence has significant homology to the conserved site in rat and mouse VASP, but is not conserved in mena or EVL proteins.

Modification Site: Thr-278

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P50552

Source: Phospho-VASP (Thr-278) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding threonine 278 in human VASP.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Actin filament tethering and bundling are important mechanisms involved in actin superstructure assembly. The ENA/VASP family includes VASP, mena, and Ena-Vasp-like (EVL). These multidomain proteins localize to the leading edge of filopodia where they associate with AFs, interact with profilin, and compete with capping proteins at the barbed end of AFs. Artificial relocalization of VASP from the plasma membrane to mitochondrial membranes inhibits filopodial formation and axon branching, while deletion of all three ENA/VASP proteins produces defects in cortical axon-tract formation. Regulation of VASP protein activity occurs through phosphorylation at Ser-157, Ser-239, and Thr-278. AMPK phosphorylates Thr-278, leading to impaired actin stress fiber assembly and changes in cell morphology.