bs-70709R

DATASHEET

Host: Rabbit

Target Protein: β-Tubulin (central region)

Specificity: This antibody detects a 50 kDa* protein corresponding to the molecular mass of β-Tubulin on SDS-PAGE immunoblots of purified brain tubulin and mouse brain tissue. This sequence is identical to similar regions in βI, βII, and βIII-Tubulin isotypes, and is well conserved in tubulins from most eukaryotic species.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: Q13509

Source: βIII-Tubulin synthetic peptide (coupled to KLH) corresponding to amino acid residues from the central region of human βIII-Tubulin.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro. Unphosphorylated Ser-444 in βIII-Tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in β-Tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.