bs-70698R

DATASHEET

Host: Rabbit

Target Protein: TRPM7 (cytoplasmic region)

Specificity: This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 cells and detects a 70 kDa recombinant C-terminal kinase domain of human TRPM7. This site is not well conserved in rat and mouse TRPM7, and has no homology to other TRPM family members.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: Q96QT4

Source: TRPM7 synthetic peptide (coupled to carrier) corresponding to amino acids from the cytoplasmic region of human TRPM7.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.