bs-70667R

DATASHEET

Host: Rabbit

Target Protein: nSMase2 (C-terminal region)

Specificity: The antibody detects a 78 kDa* band corresponding to nSMase2 on SDS-PAGE immunoblots of adult mouse brain and rat PC12 cells. This sequence is highly conserved in rat and mouse nSMase2, and has low homology to other nSMase family members.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: Q9NY59

Source: nSMase2 synthetic peptide corresponds to amino acids in the C-terminal region of human nSMase2.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Cellular stress leads to hydrolysis of sphingomyelin to generate lipid second messenger molecules including ceramide, sphingosine, and sphingosine-1-phosphate. A variety of sphingomyelinase activities have been described that differ in tissue and subcellular distribution, as well as pH and cation dependance. These enzymes generate ceramide for specific signaling pathways that lead to a wide range of cellular responses, such as apoptosis, cell cycle arrest, cell survival, and cell proliferation. Neutral sphingomyelinases (nSMases) are Mg2+-dependent neutral pH SMases, and the family includes nSMase1, nSMase2, and nSMase3. These nSMases differ in their tissue distribution and subcellular localization. nSMase2 (also known as SMPD3) is expressed primarily in brain, and co-localizes with Golgi markers in several cell lines. The activity and phosphorylation of nSMase2 is upregulated in response to oxidative stress. In addition, nSMase2 binds calcineurin, and this interaction inhibits nSMase2 activity.