bs-70654R

DATASHEET

Host: Rabbit

Target Protein: Semaphorin-3F (C-terminal region)

Specificity: The antibody detects a 95 kDa* protein corresponding to the apparent molecular mass of Sema-3F on SDS-PAGE immunoblots of adult mouse brain and liver. This sequence is highly conserved in rat and mouse Sema-3F, and has low homology to other semaphorin family members.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: Q13275

Source: Semaphorin 3F synthetic peptide (coupled to carrier protein) corresponds to amino acids from the C-terminal region of human Sema-3F.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3F (Sema-3F) is a class III secreted semaphorin that binds with high affinity to Neuropilin-2, and low affinity to Neuropilin-1. During peripheral and central nervous system development, Sema-3F has critical roles in axon guidance and dendrite outgrowth. Sema-3F has also been shown to inhibit angiogenesis by manipulating VEGFR function and decreasing blood vessel density. In cancers, Sema-3F induces a poorly vascularized, encapsulated, non-metastatic phenotype through chemorepulsion of endothelial cells in melanoma, while Sema-3F disrupts intercellular contacts of MCF7 breast cancer cells through delocalization of E-cadherin and β-catenin. Thus, Sema-3F may have important roles in axon guidance, angiogenesis, and tumor progression