bs-70653R

DATASHEET

Host: Rabbit

Target Protein: Semaphorin-3E (N-terminal region)

Specificity: The antibody detects 87 and 61 kDa bands corresponding to the full-length and furin-proccessed forms of Sema-3E in human PC-3 and rat PC12 cells. This sequence is well conserved in rat, mouse, and chicken Sema-3E, and has low homology to other semaphorin family members.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: O15041

Source: Sema-3E (N-terminal region) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminal region of human Sema-3E.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 0.05% NaN3

Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliq

Background:

The Semaphorin family of axon guidance molecules includes secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3E (Sema-3E) is a class III secreted semaphorin that binds Plexin D1 receptor, and has putative roles in axon guidance, angiogenesis, and cancers. Mutations in the Sema-3E gene are associated with CHARGE syndrome, a disorder that has nerve and cardiovascular abnormalities. The Sema-3E protein is expressed as full length (87 kDa) and furin-dependent processed forms (61 and 25 kDa). These forms are observed as monomers, as well as homo- and hetero-dimers. Sema-3E protein is frequently expressed in human cancer cell lines and in solid tumors from breast cancer patients. The expression of Sema-3E in mammary adenocarcinoma cells induces the ability to form experimental lung metastasis, and this activity requires furin-dependent production of the 61 kDa Sema-3E protein.