bs-70646R

DATASHEET

Host: Rabbit

Target Protein: Robo1 (C-terminal region)

Specificity: The antibody detects a 180 kDa* band corresponding to Robo1 in Western blots of human HepG2 and HUVEC cells, as well as mouse C2C12 cells. This sequence has significant homology to the same region in rat and mouse Robo1, and has low homology to other Robo family members.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: Q9Y6N7

Source: Robo1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human Robo1.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The Robo family of repulsive guidance receptors (Robo1-4) have important roles in controlling axon guidance and cell migration. These receptors are members of the immunoglobulin (Ig) superfamily and consist of an ectodomain with five Ig domains and three fibronectin type III repeats, a single transmembrane domain, and a long cytoplasmic tail that contains four blocks of conserved cytoplasmic sequences. In Drosophila, mutations in Robo, and its midline-expressed ligand Slit, result in too many axons crossing and staying at the midline. Several proteins that regulate the actin cytoskeleton, including cAbl, Ena, and Rho-family GTPases, contribute to the Robo signaling pathway. cAbl phosphorylates Robo1 at Tyr-1073, and this may inhibit Robo activity, while Slit-Robo signaling activates both Rac and Rho, and inactivates Cdc42. Thus, Robo guidance receptors control axon outgrowth and cell migration through activation of cell signaling pathways that regulate cytoskeletal dynamics.