DATASHEET
Host:
Rabbit
Target Protein:
C-Raf (C-terminus)
Specificity:
The antibody detects C-Raf in western blots of a purified GST-fusion protein containing human C-Raf.
Clonality:
Polyclonal
Isotype:
IgG
Swiss Prot:
P04049
Source:
C-Raf synthetic peptide (coupled to KLH) corresponds to amino acids from the C-terminus of human C-Raf. This sequence is conserved in C-Rafs from many species, and is not found in A-Raf or B-Raf.
Purification:
Antigen Affinity purification
Storage Buffer:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage:
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Background:
The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation