Host: Rabbit
Target Protein: PDK1 (Tyr-9)
Specificity: This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose, then Affinity purification using phospho-PDK1 (Tyr-9) peptide. The purified antibody detects a 65 kDa* protein corresponding to the apparent molecular mass of PDK1 on SDS-PAGE immunoblots of human A431 cells treated with EGF, IGF-1, or pervanadate, but not in untreated cells. PDK1 (Tyr-9) reactivity was also observed in adult mouse brain.
Modification Site: Tyr-9
Clonality: Polyclonal
Isotype: IgG
Swiss Prot: O15530
Source: Phospho-PDK1 (Tyr-9) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding tyrosine 9 in human PDK1. This sequence is highly conserved in rat and mouse PDK1.
Purification: Antigen Affinity purification
Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Background:
Size: 100ul
Predicted Molecular Weight: 65
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.