bs-70596R

DATASHEET

Host: Rabbit

Target Protein: Paxillin (Tyr-118)

Specificity: This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells treated with pervanadate. This reactivity is not observed after akaline phosphatase treatment. This sequence is highly conserved in rat and mouse paxillin and is also found in all isoforms (a, b, g) of paxillin.

Modification Site: Tyr 118

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P49023

Source: Phospho-Paxillin (Tyr-118) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 118 from human paxillin.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Paxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr-31 and Tyr-118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.