bs-70554R

DATASHEET

Host: Rabbit

Target Protein: Myosin 7B/MHC14 (Hinge region)

Specificity: The antibody detects a 215 kDa* protein corresponding to the apparent molecular mass of MYH7B on SDS-PAGE immunoblots of mouse extraocular muscle and brain. This sequence is highly conserved in rat and human MYH7B, and has less than 50% identity with other MYH family members.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: A2AQP0

Source: A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the hinge region from mouse MYH7B/MHC14.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11).