bs-70553R

DATASHEET

Host: Rabbit

Target Protein: Myosin 4/MyHC-IIB (C-terminus)

Specificity: The antibody detects a 215 kDa* protein corresponding to the apparent molecular mass of MYH4 on SDS-PAGE immunoblots of mouse extraocular muscle and mouse C2C12 cells. The antibody may also detect other skeletal MYHs, such as MYH1 and MYH2, which have significant homology to the C-terminal region of MYH4. This sequence has 100% identity with rat and human MYH4, and has significant homology to MYH1, MYH2, and MYH8.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: Q5SX39

Source: A synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus from mouse MYH4/MyHC-IIB.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Non-muscle myosin II is an actin-based motor protein essential to cell motility, cell division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC). In vertebrates, there are three NMHC-II isoforms (NMHC-IIA, NMHC-IIB, and NMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation of NMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, leading to activation of myosin II motor activity and increased myosin filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin. NMHC-II phosphorylation may be an important mode for regulating myosin-II assembly. PKC phosphorylates NMHC-IIA on Ser-1916 in the C-terminal region and NMHC-IIB on multiple serines in the tailpiece. Casein kinase II phosphorylates NMHC-IIA on Ser-1943 in the tailpiece and increases disassembly of NMHC-IIA filaments.