bs-70520R

DATASHEET

Host: Rabbit

Target Protein: IκBα (C-terminus)

Specificity: This antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of human A431 and Jurkat cells, as well as mouse C2C12 cells. This peptide sequence has high homology to rat and mouse IκBα, and low homology to other IκB proteins.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P25963

Source: IκBα (C-terminus) synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus of human IκBα.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.