bs-70441R

DATASHEET

Host: Rabbit

Target Protein: CXCR4 (C-terminal region)

Specificity: The antibody detects bands at 35, 42, 50, and 100 kDa* corresponding to various post-translationally modified forms of CXCR4 on SDS-PAGE immunoblots of human Jurkat and PC3 cells. These bands co-migrate with similar bands detected with anti-CXCR4 (Ser-324/Ser-325) and anti-CXCR4 (a.a. 322-329) antibodies.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P61073

Source: CXCR4 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human CXCR4.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA and 0.05% NaN3

Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliq

Background:

The chemokine receptor CXCR4 is a widely expressed G protein-coupled receptor required for development, hematopoiesis, organogenesis, and vascularization. In disease, CXCR4 has been implicated in WHIM syndrome, HIV, and cancers. Regulation of CXCR4 function occurs through phosphorylation at multiple sites in the C-terminal region. These sites have been shown to be phosphorylated after CXCL12 stimulation, and involve several kinases, such as PKC and GRK kinases. After CXCL12 stimulation of HEK293 cells, Ser-324 and Ser-325 become phosphorylated by PKC and GRK6, while Ser-330 and Ser-339 are phosphorylated by only GRK6. In human astroglia cells, Ser-324 and Ser-325 are rapidly phosphorylated in endogenous CXCR4, while Ser-330 was phosphorylated with slower kinetics. In addition, arrestin binding to CXCR4 is driven by this phosphorylation of far C-terminal residues. Thus, site-specific phosphorylation of CXCR4 may be regulated by multiple kinases lead to complex regulation of CXCR4 signaling.