bs-70430R

DATASHEET

Host: Rabbit

Target Protein: CREB (Ser-271)

Specificity: The antibody detects a 43 kDa* protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human A431, Jurkat, and rat PC12 cells treated with Calyculin A, but is not observed after lambda phosphatase treatment. The site has some homology to the conserved site in ATF1 (Ser-198) and CREM (Ser-287). This sequence is well conserved in rat, mouse, chicken, and zebrafish CREB.

Modification Site: Ser-271

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P16220

Source: Phospho-CREB (Ser-271) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-271 in human CREB.

Purification: Antigen Affinity purification

Storage Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles in cell survival and proliferation. CREB is expressed in various tissues, and has important gene-regulating roles in the nervous system. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases. CREB is phosphorylated at other sites in response to calcium influx and DNA damage. The DNA-damage responsive nuclear kinase, HIPK2, can phosphorylate Ser-271 but not Ser-133 in CREB, and this phosphorylation activates CREB transactivation function. Mutation of Ser-271 to Glu-271 potentiates the CREB transactivation function. Thus, phosphorylation of Ser-271 may be the mode of activation for CREB-dependent transcription in response to genotoxic stress.