bs-70315R

DATASHEET

Host: Rabbit

Target Protein: Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295)

Specificity: Specific for endogenous levels of the ~250 kDa and ~150 kDa splice variants of XIRP1 phosphorylated at Ser295. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Modification Site: Ser295

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 165904

Swiss Prot: O70373

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of mouse XIRP, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007). The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008). Loss of mXina (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007). Phosphorylation of Ser-295 plays a key role in XIRP1 signaling, and therefore in cardiac hypertrophy, as it has been shown that acute pressure-overloaded hearts rapidly increased phosphorylation at the Ser-295 residue of XIRP1. (Chang et al., 2013).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 150, 250

Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.