bs-70304R

DATASHEET

Host: Rabbit

Target Protein: Tyrosine Hydroxylase (Ser31)

Specificity: Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser31.

Modification Site: Ser31

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25085

Swiss Prot: P04177

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser31 of rat tyrosine hydroxylase, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 60

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.