bs-70299R

DATASHEET

Host: Rabbit

Target Protein: Tryptophan Hydroxylase (Ser58)

Specificity: Specific for endogenous levels of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser58.

Modification Site: Ser58

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24848

Swiss Prot: P09810

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of rat tryptophan hydroxylase 1 (TPH1), conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser-58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 55

Cross Reactive Species: Mouse
Rabbit

Predicted Cross Reactive Species: Human
Rat
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.