bs-70297S

DATASHEET

Host: Sheep

Target Protein: Tryptophan Hydroxylase

Specificity: Specific for endogenous levels of the ~55 kDa tryptophan hydroxylase protein.

Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P17290

Source: Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 55

Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Bovine
Sheep
Horse
Cat
Guinea Pig
Goat
Hamster
Non-Human Primate
Vole

For research use only. Not intended for diagnostic or therapeutic use.