bs-70296R

DATASHEET

Host: Rabbit

Target Protein: Troponin I (cardiac) Ser43

Specificity: Specific for endogenous levels of the ~25 kDa cardiac troponin I protein phosphorylated at Ser43. Immunolabeling is greatly decreased with λ-phosphatase treatment.

Modification Site: Ser43

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 21954

Swiss Prot: P48787

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 of mouse troponin I, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle (Noland et al, 1995). Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser-43 and Ser-45 of cardiac troponin I plays a substantial role in the PKC mediated depression. (Montgomery et al, 2002).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 25

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Bovine
Sheep
Zebrafish
Xenopus
Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.