bs-70295R

DATASHEET

Host: Rabbit

Target Protein: Troponin I (cardiac) Ser150

Specificity: Specific for endogenous levels of the ~25 kDa cardiac troponin I protein phosphorylated at Ser150. Immunolabeling is greatly decreased with λ-phosphatase treatment.

Modification Site: Ser150

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 21954

Swiss Prot: P48787

Source: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 of mouse troponin I, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser-23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser-23 and Ser-150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser-150 over Ser-23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 25

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Non-Human Primate

For research use only. Not intended for diagnostic or therapeutic use.