bs-70291R

DATASHEET

Host: Rabbit

Target Protein: TREM2

Specificity: Specific for endogenous levels of the ~28 kDa TREM2 protein.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 54209

Swiss Prot: Q9NZC2

Source: Recombinant human TREM2 protein expressed in and purified from E. Coli.

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

Triggering receptor expressed on myeloid cells 2, TREM2, is a type I transmembrane innate immune receptor predominantly expressed by microglia in the central nervous system and is genetically linked to the risk for Alzheimer’s disease (Schmid, C.D., et al., 2002). TREM2 has been shown to be a specific marker for alternatively activated microglia known as M2 cells (Cherry, J.D. et al., 2014). TREM2 binds to its adaptor, DNAX-activating protein (DAP12) on the surface of microglia to enact innate immune responses and downstream cellular responses or signaling pathways (Jay,T.R, et al., 2017). TREM2 has important roles in microglial phagocytosis of apoptotic neurons, damaged myelin, and amyloid plaques (Gratuze, M., et al., 2018). In early brain development, TREM2 is essential for synaptic pruning (Filipello, F. et al., 2018).

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 28

Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.