bs-70284R

DATASHEET

Host: Rabbit

Target Protein: TGN38/46

Specificity: Specific for endogenous levels of the ~35 kDa TGN38 protein. Additionally, a ~97 kDa band representing glycosylated TGN38 protein may be seen in rodent species.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 192152

Swiss Prot: P19814

Source: Synthetic peptide from the C-terminus region of the rat TGN38/46 protein, conjugated to keyhole limpet hemocyanin (KLH).

Purification: Antigen Affinity purification from Pooled whole antiserum

Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Background:

TGN38, also known as TGN46, is a type 1 integral membrane protein which predominantly localizes to the trans-Golgi network (TGN), and rapidly cycles to and from the cell surface. The cytosolic domain of TGN38/46 is involved in the reversible recruitment to the membrane with other cytosolic proteins for vesicle formation, while the luminal domain is involved in maintaining the morphology of the TGN (Banting, G., Ponnambalam, S., 1997). The cytosolic domain of TGN38/46 forms a complex with rab6, a small GTP-binding protein, and p62 where vesicle formation occurs at the exit sites of the TGN (Jones, S.M., et al, 1993). TGN38 is widely used as a reliable Golgi marker.

Size: 100ul

Concentration: Lot Dependent

Predicted Molecular Weight: 35, 97

Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.